Table 1.
Spermatogenic cells and DNA contribution of spermatogenic cells in prepubescent mice and fractionated adult testis cells
| Cell type (genome copies)d | Days after birtha
|
Fractionsb
|
|||
|---|---|---|---|---|---|
| 8
|
10
|
18
|
Pach
|
Hapl
|
|
| Cell(DNA) | Cell(DNA) | Cell(DNA) | Cell(DNA) | Cell(DNA)c | |
| Sertoli cells (2n) | 73(73) | 51(39) | 35(23) | −(−) | −(−) |
| Spermatogonia (2n) | 27(27) | 18(14) | 9(6) | −(−) | −(−) |
| Preleptene and leptene (4n) | −(−) | 31(47) | 14(18) | −(−) | −(−) |
| Zygotene (4n) | −(−) | −(−) | 7(9) | −(−) | −(−) |
| Pachytene (4n) | −(−) | −(−) | 34(44) | 97(99) | 20(50) |
| Haploid (n) | −(−) | −(−) | 1(−) | 3(1) | 80(50) |
a
Testis cells of prepubescent mice sacrificed at 8, 10, and 18 d of age.
b
Testis cells in purified fractions from elutriation centrifugation of testes from adult mice; Pach, pachytene fraction; Hapl, haploid fraction.
c
Data are presented as percentage of total cells in a testis cell suspension (cell) and as a percentage of total DNA prepared from the same testis cell suspension (DNA). −, no cells found among 2000 assessed testis cells.
d
The DNA contribution of the different cell types was calculated with consideration taken for the different number of haploid genome copies (n).