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. 1997 Dec;8(12):2519–2537. doi: 10.1091/mbc.8.12.2519

Figure 11.

Figure 11

dna2 mutants replicate the bulk of their DNA. Strains YDF100 (DNA2+), YDF102 (dna2–22), and tsDNA2 (Budd and Campbell, 1995) were grown to OD 0.25 in YPD and arrested at 30°C with 10 μg/ml α factor. After 3.5 h, cultures were spun down and resuspended in prewarmed (37°C) YPD+0.2 mM HU and incubated at 37°C for 3 h. Cells were then washed and resuspended in prewarmed (37°C) YPD, and 5-ml aliquots were taken and analyzed for DNA content.