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A dna2-Δ20 deletion mutant is inviable and not suppressed by Tor1p. YDF27 (dna2-Δ20/DNA2+) (A), or YDF27 transformed with pYDF113 (DNA2) (B), or pYDF77 (GAL1p→TOR1) (C) were sporulated and tetrads dissected on either YPD (A), (B), or YPGal (C), and plates were incubated at 30°C for 4 d and photographed.
