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. 1997 Dec;8(12):2519–2537. doi: 10.1091/mbc.8.12.2519

Figure 7.

Figure 7

Phenotype of the dna2–22 Ts mutant. Strains YDF100 (DNA2+) and YDF102 (dna2–22) were grown at 30°C in YPD to a density of 1.5 × 106 cells/ml, and cultures were split and incubated at either 30°C or 37°C for 4 h. Top panel, DAPI staining. Shown are DIC images with DAPI staining overlaid using Adobe Photoshop 3.0. Bottom panel, cells were processed for propidium iodide (PI) staining and flow cytometric analysis.