Table 2.
Mitotic recombination and chromosome loss in dna2 mutants
| Strain | Temperature | Recomb. (×104) | Monosomes (×105) |
|---|---|---|---|
| DNA2+/DNA2+ | 30°C | 7.48 ± 1.89 | 7.04 ± 1.43 |
| 37°C | 6.21 ± 0.83 | 16.3 ± 2.81 | |
| DNA2+/dna2-22 | 30°C | 7.96 ± 1.45 | 7.83 ± 0.52 |
| 37°C | 6.73 ± 0.38 | 16.5 ± 3.49 | |
| dna2-22/dna2-22 | 30°C | 8.75 ± 2.73 | 10.92 ± 5.3 |
| 37°C | 88.7 ± 16.1 | 183.2 ± 23.0 |
Strains YDF104 (DNA2+/DNA2+), YDF105 (DNA2+/dna2-22) and YDF106 (dna2-22/dna2-22) were grown to log phase in YPD to OD = .1 (∼3 × 107 cells/ml) and cultured at either 30°C or 37°C for 2 h. This gives ∼80% large budded arrest as assayed microscopically and results in a 50% reduction in viability as assessed by comparing microscopic counts with colony counts (our unpublished results). The frequency of mitotic recombinants and monosomic cells was determined as described (see MATERIALS AND METHODS). Numbers represent the averages and SDs from three independent experiments.