Table 3.
Effect of checkpoint mutations on dna2-22 viability and G2/M arrest
| Strain | Viability
|
%
Large
Budded
|
||
|---|---|---|---|---|
| 24°C | 37°C | 30°C | 37°C | |
| WT | 97.5 ± .94 | 96.4 ± 2.3 | 30.0 ± 5.6 | 24.0 ± 3.6 |
| mec1 | 92.3 ± 1.1 | 77.4 ± 5.8 | 29.0 ± 2.65 | 27.3 ± 2.52 |
| rad9 | 94.6 ± .49 | 94.8 ± 1.9 | 25.7 ± 1.53 | 23.3 ± 2.31 |
| dna2-22 | 94.6 ± 1.7 | 2.77 ± 1.6 | 26.7 ± 3.79 | 86.3 ± 5.13 |
| dna2-22 mec1-1 | 89.3 ± 2.0 | 3.48 ± 1.4 | 27.3 ± 0.58 | 4.50 ± 2.65 |
| dna2-22 rad9 | 93.7 ± 1.3 | 6.53 ± 2.4 | 26.3 ± 3.21 | 40.7 ± 0.58 |
| cdc2 | 92.7 ± .45 | 7.7 ± 1.5 | ND | ND |
| cdc2 rad9 | 43.6 ± .38 | .09 ± .02 | ND | ND |
| cdc13 | 92.7 ± .86 | 40.7 ± 2.9 | ND | ND |
| cdc13 rad9 | 70.6 ± 4.0 | 2.2 ± 1.9 | ND | ND |
| cdc9 | 91.2 ± 1.1 | 7.4 ± .50 | ND | ND |
| cdc9 rad9 | 92.1 ± .66 | 28.1 ± 2.7 | ND | ND |
Indicated strains were incubated in YPD at 24° to OD = .02 (approx. 8 × 106 cells/ml). Cultures were split and incubated at 24° or 37° for 4 hr. Cells were sonicated, visualized for large budded morphology, and plated on YPD at 24° for 16 hr before assaying for viability microscopically. Yeast giving rise to colonies containing >30 cells after 16 hr were considered viable. A minimum of 250 colonies was counted for each strain. Numbers represent the averages and standard deviations from 3 independent experiments.