. Author manuscript; available in PMC: 2009 Jul 15.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jun 20;870(2):233–240. doi: 10.1016/j.jchromb.2008.06.026

Table 2.

Linearity and dynamical range for comparative quantification and detection limit.

		m/z			Avg LOD [ng/ml, (pg/injection),n=5]

		d₃ labeled	non- d₃ labeled
Peak number	Derivatized estrogens			Linearity ^*
1	16-epiestriol	411.2911	408.2675	y = 0.9901x + 0.032; r² = 0.9925	2.34 (11.71)
2	16-keto-17β-estradiol	409.2611	406.2359	y = 0.9243x + 0.013; r² = 0.9953	0.39 (1.99)
3	16α-hydroxyestrone	409.2714	406.2373	y = 1.1043x + 0.010; r² = 0.9989	0.62 (3.12)
4	estriol	411.2944	408.2596	y = 0.8445x + 0.052; r² = 0.9969	0.69 (3.47)
5	17-epiestriol	411.2924	408.2642	y = 0.8582x + 0.044; r² = 0.9957	0.46 (2.34)
6	4-hydroxyestradiol	411.2786	408.255	y = 0.8376x + 0.047; r² = 0.9952	0.75 (3.75)
7	4-hydroxyestrone	409.2586	406.2433	y = 0.8411x + 0.060; r² = 0.9885	0.56 (2.84)
8	2-hydroxyestradiol	411.2882	408.258	y = 1.1139x + 0.019; r² = 0.9966	0.69 (3.47)
9	2-hydroxyestrone	409.2812	406.2363	y = 0.8512x + 0.054; r² = 0.9989	0.75 (3.75)
10	17β-estradiol	395.2286	392.1844	y = 0.8421x + 0.016; r² = 0.9988	0.44 (2.23)
11	4-methoxyestradiol	425.3775	422.3347	y = 0.8921x + 0.033; r² = 0.9987	0.48 (2.40)
12	2-methoxyestradiol	425.3782	422.3412	y = 0.8656x + 0.041; r² = 0.9988	0.37 (1.87)
13	estrone	393.2098	390.1731	y = 0.8797x + 0.039; r² = 0.9928	0.36 (1.80)
14	4-methoxyestrone	423.3415	420.3153	y = 0.8856x + 0.027; r² = 0.9986	0.62 (3.12)
15	2-methoxyestrone	423.3394	420.3125	y = 0.8934x + 0.021; r² = 0.9980	0.46 (2.34)
16	2-hydroxyestrone-3-methyl ether	423.3481	420.315	y = 0.9089x + 0.029; r² = 0.9989	0.62 (3.12)

x= Concentration ratio of non-d3 labeled to d3-labeled analytes; y= average extracted ion chromatographic peak intensity ratio of these pair of analytes. Linearity range for all is 1:1~1:40.