Real time RT-PCR analysis for the expression of SIX1, WASL, PFTK1, CAECAM1, and SPON2 mRNA in cultured human trabecular meshwork cells and human optic nerve astrocytes. For real time RT-PCR analysis, means ± standard deviation of four independent experiments run in duplicate are shown. The mean value obtained for the gene with lowest signal was set at 1.