Real time RT-PCR analysis for the expression of SIX1, WASL, PFTK1, CAECAM1, and SPON2 mRNA in cultured human trabecular meshwork cells after treatment with different amounts of recombinant myocilin (2.5ng, 5 ng, 25 ng, 50 ng, 100 ng) per ml cell culture medium. For real time RT-PCR analysis, means ± standard deviation of two independent experiments run in duplicate are shown. The mean value obtained for untreated control cells was set at 1. Asterisks mark statistical significant differences between treated cells and controls (p < 0.05 for * and p <0.02 for **). N.d.: not detectable.