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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Mol Cancer Ther. 2008 Oct;7(10):3256–3264. doi: 10.1158/1535-7163.MCT-08-0157

Figure 2.

Figure 2

A: CDDP abrogates HSF-1-mediated induction of Hsp70 and Hsp27. A: A549 cells were treated with vehicle (DMSO), 100 nM GA, 30 μM CDDP, or GA+CDDP 24 h, then washed, and incubated for times indicated from initial treatment. 50 μg of cell lysate was separated by SDS-PAGE, then probed by western blotting. Actin was used as a loading control. B: A549 cells were treated with vehicle (DMSO, lanes 1, 7), 100 nM GA, with or without increasing doses of CDDP for 24 h. 50 μg of cell lysate was separated by SDS-PAGE, then probed by western blotting. Actin is used as a loading control. C: 250 ng total mRNA from cells treated as indicated was amplified by one step RT-PCR, then separated on a 1% agarose gel. Lane 1: PCR reaction in absence of DNA, lane 2: untreated cells, lane 3: cells treated with DMSO.