Figure 1.
Special siRNA targeting ABCC2 silences the mRNA and protein expressions of ABCC2 in NPC cells. (A) Expression of ABCC2 mRNA in NP69 and human NPC cell lines by quantitative RT-PCR. N = 3, *P < 0.05 vs. NP69. (B and C)Analysis of ABCC2 expression levels in CNE2 cells treated with ABCC2-shRNA construct (ShABCC2-1 and shABCC2-2) and negative construct (ShABCC2-). (B) The expressions of ABCC2 mRNA detected by quantitative RT-PCR. (C) The expressions of ABCC2 protein detected by western blot. Data were expressed as mean ± SEM value. N = 3, *P < 0.05 vs. CNE2.