Table 5.
Expression of Wnt gene in mouse totipotent teratocarcinoma embryonic cell (F9), human embryonic stem cell (H7), and human mesenchymal cells (hM).
| gene\cell | F9 | H7 | hM |
| Wnt 1 | + | + | - |
| Wnt 2 | - | + | + |
| Wnt 2b | + | + | + |
| Wnt 3 | + | + | + |
| Wnt 3a | + | + | + |
| Wnt 4 | + | + | + |
| Wnt 5a | + | + | + |
| Wnt 5b | + | + | + |
| Wnt 6 | + | + | + |
| Wnt 7a | + | + | + |
| Wnt 7b | + | + | + |
| Wnt 8a | + | + | - |
| Wnt 8b | + | + | - |
| Wnt 9a | + | + | + |
| Wnt 9b | + | + | - |
| Wnt 10a | + | + | - |
| Wnt 10b | + | + | + |
| Wnt 11 | + | + | - |
| Wnt 16 | + | + | + |
RNA was extracted from F9, H7 and hM and reverse-transcribed to obtain first strand cDNA. Specific primers were used in PCR to amplify each Wnt cDNA. Amplified products were purified and confirmed by DNA sequencing.