Table 6.
Expression of Frizzled and LRP genes in mouse totipotent teratocarcinoma embryonic cell (F9), human embryonic stem cell (H7), and human mesenchymal cells (hM).
| gene\cell | F9 | H7 | hM |
| Fz 1 | + | + | + |
| Fz 2 | + | + | + |
| Fz 3 | + | + | + |
| Fz 4 | + | + | + |
| Fz 5 | + | + | + |
| Fz 6 | + | + | + |
| Fz 7 | + | + | + |
| Fz 8 | + | - | - |
| Fz 9 | + | + | + |
| Fz 10 | + | + | + |
| LRP5 | + | + | + |
| LRP6 | + | + | + |
RNA was extracted from F9, H7 and hM and reverse-transcribed to obtain first strand cDNA. Specific primers were used in PCR to amplify each Wnt cDNA. Amplified products were purified and confirmed by DNA sequencing.