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. 1997 Dec;8(12):2539–2551. doi: 10.1091/mbc.8.12.2539

Figure 4.

Figure 4

Deletion of CPP1 in C. albicans. (A) Deletion strategy and restriction map of the CPP1 gene. PCR with the divergent oligodeoxynucleotides O24 and O25 was used to delete the PTP-active site region (ptp) of CPP1. A hisG-URA3-hisG cassette was then inserted. Restriction sites are as follows: B, BamHI; Bg, BglII; C, ClaI; H, HindIII; Nc, NcoI; Ns, NsiI; Sp, SpeI. (B) Southern blot analysis of CPP1 disruptions with a HindIII-SpeI CPP1 fragment as a probe. Genomic DNA samples were digested with HindIII from the following strains: lane 1, CAI4 (ura3/ura3 CPP1/CPP1); lane 2, CP29 (ura3/ura3 CPP1/cpp1Δ::hisG-URA3-hisG); lane 3, CP29–1(ura3/ura3 CPP1/cpp1Δ::hisG): lane 4, CP29–1-7 (ura3/ura3 cpp1Δ::hisG-URA3hisG/cpp1Δ::hisG), and lane 5, CP29–1-7 L4 (ura3/ura3 cpp1Δ::hisG/ cpp1Δ::hisG).