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Colonies of C. albicans cells grown on solid Spider medium containing glucose. Strain SC5314 (wild type); cpp1 null mutant CP29–1-7 (cpp1Δ/cpp1Δ::URA3); cpp1 null mutant into which the CPP1 gene has been replaced by homologous recombination CP29–1-7RI (cpp1Δ/cpp1Δ::CPP1-URA3). (A) Cells grown for 5 d at 37°C. (B) Cells grown for 8 d at 23°C. (C) Agar-penetrating growth remaining after surface washing of colonies grown for 8 d at 23°C from B (2× objective). Bars, 2 mm.
