Figure 7.
Virulence assays of BALB/c mice infected i.v. with either cpp1 null mutant (cpp1Δ/cpp1Δ::URA3) or CPP1 reintegrant (cpp1Δ/cpp1Δ::CPP1-URA3) C. albicans strains. (A) Survival curves of mice (n = 11) injected with 5 × 105 cells. (B) Fungal burden of infected tissues. (C) Fluorescence micrographs of Calcofluor white-stained hypha of C. albicans purified from KOH-solubilized kidneys 2 d after infection. a, CP29–1-7RI (cpp1Δ/cpp1Δ::CPP1-URA3); b, CP29–1-7 (cpp1Δ/cpp1Δ::URA3). Bar, 15 μm. Our unpublished results showed that survival curves of mice injected with the wild-type strain SC5314 and the CPP1 reintegrant were identical.