Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primer sequences and optimized annealing temperatures for each pair of primers
| Primers | Sequence | CpG sites | Location | Length (bp) | Annealing temp. (°C) |
| P1 | 5' TATGTAGGATCGAAGTTTTC 3' | 2 | -405 ~ -64 | 341 | 60–62 |
| P2 | 5' TAAAAACGCCTCCGCCG 3' | 3 | |||
| P3 | 5' GGGTATAGTGCGGGAGC 3' | 2 | -235 ~ +22 | 257 | 65–67 |
| P4 | 5' CAACCGCACGAAAAACGC 3' | 3 | |||
| P5 | 5' GAGGCGTTAGGCGGTTC 3' | 3 | -180 ~ -26 | 155 | 64.5 |
| P6 | 5' AACTAACGACCGACGAAC 3' | 3 | |||
| U1 | 5' GAGGTGTTAGGTGGTTTGTT 3' | 3 | -180 ~ +71 | 252 | 62 |
| U2 | 5' CCCACCAAAATCACAACAA 3' | 3 |
