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. Author manuscript; available in PMC: 2009 Aug 15.
Published in final edited form as: J Immunol. 2008 Aug 15;181(4):2465–2471. doi: 10.4049/jimmunol.181.4.2465

Figure 1. Transwell-based electrotaxis assay.

Figure 1

(A) Illustration of transwell assay for studying cell migration in an applied electric field. An electric field was applied to the transwell (2.5V across the transwell) by placing two platinum electrodes, which were connected to a DC power supply, to the top and bottom well of the transwell respectively. Cells were allowed to migrate from the top well to the bottom well through the membrane. The electrical potential in the transwell assay was simulated. (B) The simulated electrical potential was plotted as a function of normalized vertical coordinate Y for different normalized horizontal coordinate X within the membrane (−0.3–0.3). 1 = 7.8mm for the normalized coordinate X. 1 = 10mm for the normalized coordinate Y.