Figure 1.

Guanine nucleotides influence the distribution of dynII on coated pits in A431 cells. Upper panels show coated pits with various degrees of curvature detected at steady state on ripped-off plasma membrane preparations from perforated A431 cells. Endogenous dynII was detected using hudy-1 mAb followed by 10 nm gold-conjugated secondary antibodies. Dynamin is uniformly distributed on flat lattices (a, b) and on curved coated pits (b–d) but is localized peripherally at the base around the more deeply invaginated coated pits (e, f) distinguished by the pool of stain encircling their base. Lower panels show representative fields from perforated A431 cells incubated in the presence of GTP (g), GTPγS (h), or GDPβS (i). ∼39,000×.