Figure 4.

DynII has a higher endogenous rate of GTP hydrolysis than dynI. Intrinsic GTPase activities of dynI (□) and dynII (○) were measured in 20 μl of PH buffer as described in EXPERIMENTAL PROCEDURES and containing 0.2 μg of dynamin and 0.25 mM GTP. Backgrounds obtained without dynamin have been subtracted. A representative experiment is shown; average rates determined from four independent experiments are given in the text.