Figure 6.

DynII shows a greater propensity for self-assembly than dynI. Light scattering measured at A_{320 nm} was used to follow dynamin self-assembly as described in EXPERIMENTAL PROCEDURES. DynI (□) or dynII (○) at 2 mg/ml in HCB150 was diluted to the indicated protein concentrations in PH buffer in the absence (A) or presence (B) of 250 μM GTP. Assays were in a final volume of 200 μl of PH buffer, fixed at 50 mM ionic strength. Steady-state assembly was reached after 20 min even at low protein concentrations, at which point the absorbance values shown were recorded. Care was taken at higher protein concentrations that readings of light scattering were taken well before complete hydrolysis of the GTP. Each point represents the mean derived from three independent determinations with all standard deviations smaller than the symbols. Data are representative of three separate experiments.