Table 1.
Primer sequences and PCR conditionsa
| Gene | Primer | Product Size (bp) | Annealing Temperature/Time (°C/s) | Extension Temperature/Time (°C/s) |
|---|---|---|---|---|
| GAPDH (NM_017008) |
F: 5′-TGAACGGGAAGCTCACTGG-3′ R: 5′-TCCACCACCCTGTTGCTGTA-3′ |
307 | 63/5 | 72/10 |
| Renin (NM_012642) |
F: 5′-TTGTGTGAGGAGGGCTGTAT-3′ R: 5′-TGCTGAGAGTGTAGGTCCTG-3′ |
199 | 58/5 | 72/8 |
| p22phox (NM_024160) |
F: 5′-TCCACTTACTGCTGTCCGT-3′ R: 5′-TCAATGGGAGTCCACTGCT-3′ |
127 | 62/5 | 72/6 |
| gp91phox (NM_023965) |
F: 5′-TGGTGATGTTAGTGGGAGC-3′ R: 5′-CTTTCTTGCATCTGGGTCT-3′ |
196 | 63/5 | 72/10 |
| Type I collagen (col1a1: Z78279) |
F: 5′-TCACCTACAGCACGCTTG-3′ R: 5′-GGTCTGTTTCCAGGGTTG-3′ |
245 | 56/5 | 72/10 |
| Type IV collagen (col4a3: L47281) |
F: 5′-GAGGGTGCTGGACAAGCTCTT-3′ R: 5′-TAAATGGACTGGCTCGGAATTC-3′ |
67 | 62/5 | 72/4 |
a
GAPDH, glyceraldehyde-3-phosphate dehydrogenase.