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X4L4 does not stimulate WRN helicase. Helicase assays. WRN (lanes 2–5) or BLM (lanes 8–11) and increasing amount of X4L4 (lanes 3–6 and 9–12) were preincubated on ice. DNA substrate (37A/37B) was added to the proteins and incubated. Products were analyzed by nondenaturing PAGE.