Table 1.
Summary Showing the Total Number of Animals (N), Numbers of Hemispheres for Optical Imaging (OI) and the Numbers of Hemispheres and Neurons Used for Single Unit Electrophysiology (Units) in Each Group
| OI |
Units Neuronal Properties |
|||||
|---|---|---|---|---|---|---|
| N | Mean age | RH | LH | RH (cells) | LH (cells) | |
| VEH | 5 | 34.6 | 5 | 5 | 4 (120) | 4 (120) |
| Zolpidem (10 mg/kg) | 6 | 32.3 | 5 | 5 | 5 (150) | 5 (150) |
| Triazolam (0.1 mg/kg) | 4 | 35.5 | 4 | 4 | 3 (90) | 3 (90) |
| Triazolam (1 mg/kg) | 1 | 28 | 1 | 1 | 1 (30) | 1 (30) |
| Total Triazolam | 5 | 34 | 5 | 5 | 4 (120) | 4 (120) |
| Ramelteon (0.1 mg/kg) | 4 | 36 | 3 | 4 | 4 (60) | 3 (90) |
| Ramelteon (1 mg/kg) | 1 | 31 | 1 | 1 | 1 (30) | 1 (30) |
| Total Ramelteon | 5 | 35 | 4 | 5 | 5 (90) | 4 (120) |
For OI, the number of imaged hemispheres is shown. In the ramelteon group, one hemisphere was not imaged due to vascular artifacts and tissue damage. In the zolpidem group, one animal was included in the sleep analyses, but excluded from plasticity assays because of clouding of the vitreous in one eye. For electrophysiology, the subset of analyzed hemispheres and the corresponding units in brackets are shown. 30 single neurons (sampled using a systematic randomization procedure) per hemisphere were used for neuronal properties analysis (see Materials and Methods). There was no difference in age between any of the groups (1-way ANOVA, ns). All animals shown were used in sleep analyses.