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. 2008 Aug 19;112(9):3671–3678. doi: 10.1182/blood-2008-05-157016

Figure 4.

Figure 4

The breadth and effector phenotype of elicited influenza-specific CD8+ T cells. Vaccination as indicated in figure panels. Single-cell suspensions from tissues harvested at day 14 after vaccination were stimulated for 8 hours with indicated peptides and antibodies against CD28 and CD49d. The frequency of IFN-γ–secreting CD8+ T cells by flow cytometry. (A-D) Spleen analysis. (A) Representative experiment. (B) IFN-γ–secreting CD8+ T cells (mean ± SEM, n = 3) after stimulation with indicated peptides. (C) Analysis after vaccination with TIV or TT. (D) The frequency of IFN-γ–secreting CD8+ T cells (mean, n = 4) specific to indicated peptides. (E) Blood: specific CD8+ T cells binding FluM1 and NS1 tetramer in humanized mice vaccinated with LAIV or TIV. (F) Blood and lungs of TIV or TT-vaccinated mice. IFN-γ and CD107a expression by CD8+ T cells in response to HIVgag or FluM1 peptides.