Figure 5.

Rate of incorporation of FN into DOC-insoluble matrix in the presence or absence of Mn²⁺. Quantitative immunoblot analysis was performed on DOC-insoluble and -soluble extracts isolated from CHOα5 cells incubated with 25 μg/ml pFN alone (open circles), 25 μg/ml with 1 mM MnCl₂ (closed circles), or 50 μg/ml pFN alone (open triangles) over the indicated periods of time. Accumulation of total cell-associated FN (A), DOC-insoluble FN matrix (B), and DOC-insoluble FN as percentage of total (C) was determined. Data are from a single experiment and are representative of resuts from at least four independent experiments as described in MATERIALS AND METHODS.