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FN(syn−) assembly in response to Mn2+ addition. Twenty-five micrograms per milliliter FN(syn−) were added to CHOα5 cells along with no MnCl2 (A) or 0.2 mM MnCl2 (B) and incubated for 16 h. Cells were then fixed and FN fibrils were detected by immunofluorescence. Bar, 10 μm.
