Figure 4.

Translocation of YFP-tagged p67^phox and p40^phox during phagocytosis of IgG-zymosan in the presence and absence of wortmannin. Time-lapse confocal microscopy was used to monitor phagocytosis of IgG-zymosan by PLB-985 neutrophils expressing YFP-tagged p67^phox or p40^phox (Videos S1–S4). N shows the location of nucleus and asterisks indicate the IgG-zymosan phagosomes monitored. Bar represents 5 μm. (A,B) PLB-p67YFP and PLB-YFPp40 cells. The relative fluorescence intensity compared with the cytosol for 5 phagosomes at indicated stages is shown in the graphs, as mean plus or minus SE. Internalized indicates 200 or more seconds after phagosome closure. (C,D) PLB-p67YFP and PLB-YFPp40 neutrophils pretreated with 100 nM wortmannin before incubation with IgG-zymosan. N shows the location of nucleus and asterisks indicate the IgG-zymosan phagosomes monitored. Bar represents 5 μm. The relative fluorescence intensity compared with the cytosol for 5 to 7 phagosomes at indicated stages is shown in the graphs, as mean plus or minus SE. Internalized indicates 120 or more seconds after phagosome closure.