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. Author manuscript; available in PMC: 2009 Aug 2.
Published in final edited form as: DNA Repair (Amst). 2008 Jun 13;7(8):1276–1288. doi: 10.1016/j.dnarep.2008.04.007

Fig. 2.

Fig. 2

The NI-modified DNA templates prepared for transcriptional assays. The vector (white) is comprised of the cloned T7 promoter and the CMV promoter/enhancer (black boxes). The tail is formed by annealing a 96-mer, a 90-mer, and an 11-mer (grey). The NI position is indicated by a black triangle. The arrowheads designate the +1 transcription start site. The size of the template is 1,140 base pairs. The expected sizes of the transcripts obtained during transcription by T7 RNAP and hRNAPII are indicated below the template.