Represents mean affective behavior for young, gonadectomized (GDX) mice administered vehicle control (white bar), E2 (horizontally-striped bar), T (black bar), or 3α-diol (gray bar) in the open field (upper left panel), elevated plus maze (upper right panel), dark–light transition (middle left panel), zero maze (middle right panel), mirror maze (lower left panel), and forced swim (lower right panel) tasks. *Denotes significant difference (P<0.05), #denotes significant trend (P<0.10).