Table 3. Trypanosome sequences obtained from the Christmas Island rats.
Species designation | Sample number | A | B | |||||||||
1a | 4 | 58 | 9–15 | 18 | 21–23 | 26 | 29 | 1–5 | 7–35 | 38–42 | ||
Trypanosoma lewisi | AJ223566 | C | A | TTCT | - - - - -TT | G | - - - | C | T | TTTTT | G -14 bp del- TCCTCGCAAGAGGT | TTTTA |
Trypanosoma musculi | AJ223568 | . | . | . . . . | TT - - - - - | . | - - - | . | . | . . . . . . | GTCCTC GCA - AGAGGT | - - - - - |
Trypanosoma cruzi | M31432 | . | G | TTTT | TT - - - A - | . | TAG | . | . | . . . . . . | TCTTCGTTTTTTTT ACGGCGAGGA | TTTAA |
Bodo saliens | AF174379 | . | T | TC- - | - - - - - A A | . | - - - | . | C | CGTTG | CTTT ACCG | AATAT |
Bodo saltans | AF208889 | T | T | CT- - | - - - - - - A | A | - - - | A | . | TACTG | CCTTC GGG | GTCAA |
Rattus rattus | E2079 | . | . | . . . . | . . . . . . . | . | . . . | . | . | . . . . . | . . . . . . . . . . . . . . . . . . . . . . . . . . . . . | . . . . . |
Putative hybrids | 18607 | . | . | . . . . | . . . . . . . | . | . . . | . | . | . . . . . | . . . . . . . . . . . . . . . . . . . . . . . . . . . . . | . . . . . |
E2074d | nd | nd | nd | nd | nd | nd | nd | nd | . . . . . | . . . . . . . . . . . . . . . . . . . . . . . . . . . . . | . . . . . | |
E2075d | . | . | - - -T | . . . . . . . | . | . . . | . | . | nd | nd | nd | |
Rattus macleari | E2077d | nd | nd | nd | nd | nd | nd | nd | nd | . . . . . | . . . . . . . . . . . . . . . . . . . . . . . . . . . . . | . . . . . |
18846 | . | . | . . . . | . . . . . . . | . | . . . | . | . | . . . . . | . . . . . . . . . . . . . . . . . . . . . . . . . . . . . | . . . . . |
Numbering begins from the first base after the 5′ PCR primer. T. lewisi is used as a reference sequence. Identities are shown as dots and differences as the differing bases except for long stretches of insertions or deletions where the entire stretch of sequences are shown. Long deletions are given as numbers of deleted bases.
A and B refer to the individual PCR products amplified with the two independent PCR primers (see Table 1).
Sequences not determined are shown as “nd”.
The results for these samples were not replicated.