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Frequency domain dynamic fluorescence depolarization of ribosome-associated apoMb and PIR90 nascent chains generated in a wild-type E. coli cell-free system. ApoMb57 displays both 2- and 3-component dynamics. Data for a control sample of ribosome-released ApoMb153 are also shown. The cartoons on the far right highlight the proposed motions (in red) associated with each fluorescence phase. The BODIPY-FL fluorophore is shown in green.
