NOS enzyme activities in EDL muscle in control and septic wild type and nNOS−/− mice, at 6–7 hrs post-FIP. In wild type mice, sepsis, or sepsis plus ascorbate injection at 6 hrs post-FIP, did not alter constitutive cNOS activity (i.e., total of eNOS + nNOS), while iNOS activity was negligible in the EDL muscle of all mice. Genetic deletion of nNOS dramatically reduced cNOS activity in control and septic muscles, indicating the constitutive NOS activity in wild type muscle was dominated by nNOS. n = 4 or 5 muscles from 4 or 5 mice per group.