Effect of ascorbate bolus (10 mg/kg, given at 6 hrs post-FIP) on plasma ascorbate concentration at 7 hrs post-FIP and on survival at 24 hrs, in wild type mice. Panel A: Control and septic mice were injected iv with saline or ascorbate at 6 hrs, and blood samples were collected at 7 hrs post-FIP. Analysis by two-way ANOVA revealed no significant effect of sepsis or ascorbate on plasma ascorbate concentration at 7 hrs (n = 5 and 6 for saline injected control and septic mice, and 5 and 7 for ascorbate injected control and septic mice, respectively). Panel B: Septic mice were monitored every 6 hrs for 24 hrs. Based on Kaplan-Meier survival analysis, survival of mice given ascorbate at 6 hrs post-FIP (n = 32) was significantly higher (p < 0.05) than that of mice given saline (n = 31).