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spc110–221 defect is checked by MAD1. Asynchronous cultures of an spc110–221 strain (▪) and an spc110–221 mad1Δ strain (•) were shifted to the nonpermissive temperature of 37°C at t = 0 h. At regular intervals, aliquots were removed, sonicated, and titered for colony-forming units at 21°C on YPD. A mad1Δ strain behaved like the wild-type control strain (our unpublished observations).
