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. 2008 Oct 7;105(41):15726–15731. doi: 10.1073/pnas.0804766105

Fig. 3.

Fig. 3.

Size-exclusion chromatography analysis of hOR17-4 aggregation states. (A) We used Superdex 200 GL 5/150. SEC analysis in different detergents, running buffer: SEC buffer [25 mM Tris·HCl (pH 7), 150 mM NaCl, 10% glycerol) supplemented with 3× CMC cyclofos 5 (gray line), Tri-mix (gray dashed line), 10× CMC FC14 (black line), and SEC buffer only (black dotted line). The arrow indicates the void volume. (B) Monitoring the effect of hOR17-4 aggregation states in different buffers with different pH, NaCl concentration, and reducing agent. (C) SDS/PAGE of the elution fractions from affinity purification with the same buffers as in B.