Fig. 1.
Mitochondrial depolarization causes Drp1-dependent fission. (A and B) Changes in mitochondrial fluorescence of TMRM or calcein. HeLa cells loaded with calcein/Co2+ or with TMRM were treated where indicated (arrows) with 20 μM ArA (A) or 2 μM FCCP (B). Fluorescence intensities were normalized to the initial value for comparative reasons. Data represent mean ± SE of four independent experiments. Where indicated, cells were pretreated with CsA or with the inactive analogue CsH (18). (C and D) Representative confocal images of mtRFP fluorescence in HeLa cells before (left) or 30 min after (right) treatment with 20 μM ArA (C) or 2 μM FCCP (D). Where indicated, cells were pretreated with CsA or cotransfected with K38A Drp1 (Drp1DN) (Scale bar, 20 μm).