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Aire protein levels do not vary among strains. Flow cytometric analysis of Aire expression in MECs of different genetic backgrounds (Aire vs MHC-IIhi profiles of gated CD45−G8.8+Ly51intMHC-IIhi MECs; representative of three independent mice for each inbred strain). Numbers are the mean percentage of Aire+ cells among class IIhi MECs, and the mean anti-Aire fluorescence intensity, ± SD, of Aire staining.
