Fig. 5.

Western blot analysis of proteins eluted from a Ni-NTA-agarose column after disruption of cross-linked carrier-state particles carrying N-terminal His tag YajQ. The first column shows proteins reacting with antibodies to YajQ. The second column shows proteins reacting with antibodies to P1, and the third column shows proteins reacting with antibodies to P4. Molecular mass standards positions are shown to the right, but the major P1 band has a molecular mass of 85 kDa. A, B, and C refer to presumptive cross-linked bands of YajQ and P1.