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. 2008 Oct 3;105(41):16045–16050. doi: 10.1073/pnas.0804106105

Fig. 1.

Fig. 1.

Coexpression of μ and δ receptors leads to loss of cell surface μ receptors in HEK293 cells. (A) Cell surface expression of receptors shows that coexpression with myc-tagged δ receptors induces a large retention of FLAG-tagged μ receptors in an intracellular compartment. Coexpression of HA-tagged α2AR with μ receptor or δ receptor with FLAG-tagged κ does not lead to intracellular retention supporting the specificity of μ-δ interactions. (B) Quantitation of cell surface expression of receptors shows that coexpression with myc-tagged δ receptors but not with HA-tagged α2A receptors induces a large decrease in FLAG-tagged μ receptor expression. Coexpression of δ receptor does not alter the cell surface expression of FLAG-tagged κ receptors in HEK293 cells. (C) Quantitation of cell surface expression of δ receptors cotransfected with μ receptors in Neuro2A cells. Values are expressed as mean ± standard error of the mean (SEM) (n = 3).