Table 1.
[NaCl], mM | konstraight, (M·s)−1 | lsleff, bp | , bp | Rtheory | Rmeasured |
---|---|---|---|---|---|
0 | 0.8 × 108 | 97 | 518 | 1.11 | 1.3 |
25 | 1.0 × 108 | 123 | 485 | 1.15 | 1.1 |
100 | 1.0 × 108 | 122 | 120 | 1.63 | 1.7 |
150 | 0.09 × 108 | 9.1 | 80 | 1.10 | 1.3 |
The measured on rates for extended DNA, konstraight, are used as input for the theory to obtain the values of lsleff assuming that the estimate xact = 0.75% holds for all [NaCl].