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Interaction of wild-type and E1-Ran with p10, assayed by His*Tag resin binding. His*Tag-wild-type Ran (predominantly nucleotide free, see MATERIALS AND METHODS), His*Tag-wild-type Ran·GDP, and His*Tag-E1-Ran·GDP, bound to Ni2+ resin, were incubated with p10, and the amounts of the latter bound to the resin were determined as described in MATERIALS AND METHODS. Mobility of a size marker is indicated on the left and mobility of p10 on the right. B, bound p10; U, unbound p10.
