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. 1997 Dec;8(12):2591–2604. doi: 10.1091/mbc.8.12.2591

Figure 6.

Figure 6

(A) Effects of wild-type and E1-Ran·GDP on nuclear protein import in digitonin-permeabilized buffalo rat liver cells. Assays were carried out at 21°C as described in MATERIALS AND METHODS on permeabilized cells supplemented with 1.4 mg/ml of Xenopus fraction A (which contains karyopherin α and β), 1.5 μg/ml of purified human nuclear import factor p10, the indicated concentrations of wild-type Ran·GDP (▪) or E1-Ran·GDP (○), 5 μg/ml of NLS-tagged, rhodamine-labeled human serum albumin, and 1 mM GTP. Protein import was measured as mean nuclear fluorescence in arbitrary units. (B) Inhibition of nuclear import by E1-Ran. Assays were performed at 21°C, in the presence of 1.45 mg/ml Xenopus fraction A, 1.5 μg/ml p10, 50 μg/ml wild-type Ran·GDP, 1 mM GTP, and the indicated concentrations of additional wild-type Ran·GDP (▪) or E1-Ran·GDP (○).