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Effect of E1-Ran on docking of import substrate at the nuclear pore. Permeabilized cells were incubated with complete import mixture (Mix) (karyopherins α and β (Xenopus fraction A), p10, 50 μg/ml wild-type Ran·GDP, and 1 mM GTP) plus an additional 100 μg/ml wild-type Ran·GDP or 100 μg/ml E1-Ran·GDP. Assays were performed at 21°C or at 4°C as described in MATERIALS AND METHODS.
