Figure 11.

Influence of amino acid starvation (No AA) or saturation of SNAT2 with 10 mM MeAIB on the pH sensitivity of PI3K lipid kinase activity in L6-G8C5 myotubes. Cells were incubated for 2 h at the specified pH with or without amino acids or MeAIB. All media contained serum-free MEM with 100 nM insulin. For No AA cultures, this 2-h incubation was preceded by 2 h in MEM without amino acids and with 2% dialyzed FBS. (A) Representative PI3K assay autoradiograph. (B) Pooled quantification data obtained by densitometry of three independent experiments performed as in A. *P < 0.05 versus the corresponding pH 7.4 control.