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Effect on PKB activation in L6-G8C5 myotubes of 2-h incubations at the specified pH with or without 10 mM MeAIB as in Figure 6. All media contained serum-free MEM with 100 nM insulin. (A) Representative immunoblot showing PKB activation assessed from phosphorylation of PKB at Ser 473. (B) Pooled quantification data from 18 independent experiments performed as in A expressed as percentage of the control value at pH 7.4. *P < 0.05 versus the pH 7.4 control.
