Figure 6.

(A) WT podocytes demonstrated actin stress fibers when preincubated with 10% normal human plasma for 12 to 24 h. (B) The addition of Hx (0.05 mg/ml for 30 min) was not associated with dramatic actin reorganization. (C) When WT podocytes were treated with the serine protease inhibitor AEBSF (0.5 mM) and Hx (0.05 mg/ml) for 30 min, there was minimal actin reorganization. At higher concentrations, AEBSF (1.0 mM) had independent effects on the actin cytoskeleton (D). Images are representative of n = 4 independent experiments.