Figure 9.

WT podocytes were treated with an antibody to the lectin WGA to detect the sugar moieties of the glycocalyx. (A) Cells in SFM demonstrated abundant WGA binding. (B) By contrast, cells treated with hemopexin (0.05 mg/ml for 30 min) demonstrated a reduction in WGA binding. (C and D) The comparative fluorescence intensity is shown for podocytes (C) and for glomerular endothelial cells (D) for n = 3 to 4 independent experiments showing a significant differences in WGA binding (P = 0.002 and P = 0.003, respectively, by unpaired t test).