Table 1.
Antibodies used for immunohistochemistrya
| Antibody | Dilution | Species | Reference or Supplier |
|---|---|---|---|
| α-actinin-4 | 750 | Rabbit anti-human | 33 |
| cd2ap | 2500b | Rabbit anti-mouse | 34 |
| Desmin (clone D33) | 400b | Mouse anti-human | Dako (Carpenteria, CA) |
| Ezrin (clone TB4) | 1000b | Mouse anti-chick | 35 |
| S100A4/FSP-1/MTS1 | 200 | Rabbit anti-human | Dako (Carpenteria, CA) |
| Gelsolin | 500b | Rabbit anti-mouse | 36 |
| Moesin (Q480) | 75b | Rabbit anti-human | Cell Signaling (Danvers, MA) |
| Nephrin | 1000 | Rabbit anti-mouse | 37 |
| Nestin | 10 | Mouse anti-rat | DSHB, University of Iowa |
| PAX2 | 300b | Rabbit anti-mouse | Covance (Berkeley, CA) |
| CD31/PECAM (clone MEC13.3) | 100 | Rat anti-mouse | BD Pharmingen (San Jose, CA) |
| Podocalyxin | 1000 | Rabbit anti-human | 38 |
| Podocin | 2000 | Rabbit anti-human | 39 |
| Smooth muscle actin (clone 1A4) | 200 | Mouse anti-human | Sigma (St. Louis, MO) |
| Synaptopodin | 300 | Mouse anti-rat | 40 |
| WT1 | 150 | Rabbit anti-human | Santa Cruz Biotechnology (Santa Cruz, CA) |
a
The following goat antibodies were used for detection: Cy3-conjugated anti-rabbit or anti-rat IgG (1:500), and Alexa488-conjugated anti-mouse IgG1 (1:200, Invitrogen). DSHB, Developmental Studies Hybridoma Bank; PECAM, platelet-endothelial cell adhesion molecule.
b
Paraformaldehyde fixation.