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. Author manuscript; available in PMC: 2008 Oct 27.
Published in final edited form as: Kidney Int. 2004 Mar;65(3):972–981. doi: 10.1111/j.1523-1755.2004.00476.x

Fig. 5. Renal cortical mitogen-activated protein kinases (MAPKs) activities in Dahl salt-sensitive (DS) rats.

Fig. 5

Renal cortical tissues were harvested, lysed, and used for subsequent analyses. The activities of (A) extracellular signal-regulated kinases (ERK) 1/2, (B) c-Jun NH2-terminal kinases (JNK), (C) p38 MAPK, and (D) Big MAPK-1 (BMK1) were measured as described in Methods. All values were normalized by arbitrarily setting the densitometry of DS/L rats to 1.0. Densitometric analysis of the immunoreactive bands showed that a high salt diet markedly increased renal cortical ERK1/2, JNK, and BMK1 activities in DS rats, whereas p38 MAPK activity was unchanged. Candesartan (C) treatment significantly reduced ERK1/2, JNK, and BMK1 activities by -45%, -60%, and -70%, respectively, in DS-rats. On the other hand, no differences in the amounts of ERK1/2, JNK, p38 MAPK, and BMK1 were observed in samples by Western blotting analyses using pan-ERK1/2, JNK, p38 MAPK, and BMK1 (ERK5) antibodies (data not shown).